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Project Leader: Keith Perry, Department of Plant Pathology
Cooperators: Stewart Gray, USDA-ARS & Department of Plant Pathology
Walter De Jong, Department of Plant Breeding
Type of Grant: Pest Resistant Crops
Project Locations: New York, Maine, and nationally
Abstract:
In 2002, two viruses that are part of an emerging potato disease
complex (tuber necrosis) were first described in the US. Viruses can
be difficult to score visually, compromising seed certification programs.
The focus of this proposal is: i) the collection of Potato Virus
Y (PVY) field isolates representative of those compromising potato
production, and ii) testing commercial varieties and breeding lines
to assess their resistance phenotypes. Plants from commercial production
areas were analyzed to determine which of the common viruses are present,
namely, Potato Virus A (PVA), Potato Virus M (PVM), Potato
Virus S (PVS), Potato Virus X (PVX), PVY or Potato Leafroll
Virus (PLRV). Of the 500 plants sampled, 386 contained PVY, most
commonly with one or more other viruses. Twenty one of the commercial
varieties can be inferred to be PVY susceptible. Five of the samples
contained "necrotic isolates" of PVY that induced a veinal necrosis
when inoculated onto tobacco. Two of these necrotic PVY isolates were
not recognized by the diagnostic monoclonal antibody used in Foundation
Seed programs, and three of the isolates were recognized by the diagnostic
monoclonal antibody, but did not give rise to a veinal necrosis. These
results emphasize shortcomings in the accepted methods of disease management.
We now have in hand representative field isolates of PVY. This will
allow us to assess the resistance phenotype of potato breeding lines
and commercial varieties.
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